Tumor cell detection method using complement-mediated cytolytic reaction and imaging sensor system |
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Authors: | Suzuki Masayasu Watamabe Nobuyuki Tamiya Eiichi Kataoka Tetsuro Tokunaga Tohru Karube Isao |
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Affiliation: | (1) Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta-cho, 227 Midori-ku, Yokohama, Japan;(2) Department of Cellular Immunology, National Institute of Health, Kami-Oosaki, Shinagawa-ku, 141 Tokyo, Japan |
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Abstract: | A novel tumor-detection system consisting of complementmediated cytolytic reaction and an image processing system was developed for the simple and rapid determination of tumor cells. The present system consists of a CCD image sensor, image memory board, personal computer, and microscope. When monoclonal antibody 3C4, which is specific to the guinea pig hepatoma L-10, was added to cell suspension, only L-10 cytolysis occurred. Cytolysis caused a decrease in brightness of the cells observed by phase-contrast microscopy. The phase contrast image of the cells before cytolysis was converted to a digitalized signal and stored in computer memory. After cytolysis, a brightness threshold above that of lysed cells was subtracted from the digitalized signal and compared to the signal stored before reaction. L-10 cells in mixed cell suspension were determined specifically by the system. Measurement time was only 2 sec and overall time, including reaction time, was approximately 30 min. Since this method does not require a cell washing process, automation of the whole system is possible. |
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Keywords: | Hepatocarcinoma cell monoclonal antibody complement cytolytic reaction imaging sensor system phase-contrast microscope |
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