Analysis of sterols in vegetable oils using off-line SFC/capillary GC-MS |
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Authors: | A Medvedovici F David P Sandra |
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Institution: | (1) Department of Organic Chemistry, University of Gent, Krijgslaan 281 S-4, 9000 Gent, Belgium;(2) Research Institute for Chromatography, Kennedypark 20, 8500 Kortrijk, Belgium |
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Abstract: | Summary The analysis of sterols in vegetable oils by off-line SFC followed by capillary GC-MS is described. The fractionation of the
sterols from the complex oil matrix is achieved by SFC on aminopropyl silicagel in less than 8 minutes. Injection and collection
of the sterol fraction is fully automated and time controlled. The sterols are analysed without derivatisation by capillary
GC-MS. Identification is performed by full scan electron ionisation and quantitation is carried out by extracted ion chromatography
at m/e 107, with cholesterol as internal standard. The analyses of the sterols from the sunflower oil and two olive oils illustrate
the possibilities of the method. |
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Keywords: | Supercritical fluid chromatography Capillary GC-MS Off-line fractionation Sterol fraction Vegetable oils |
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