Development and validation of rapid HPLC method for determination of doxofylline in bulk drug and pharmaceutical dosage forms |
| |
Authors: | Ashu Mittal Shikha Parmar |
| |
Institution: | (1) Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram, Kerala, India; |
| |
Abstract: | A simple, selective, rapid, and economical reversed phase high performance liquid chromatography(RP-HPLC) method for the determination of doxofylline in the commercial dosage form has been developed and validated. The separation
and quantification were achieved on an HiQ Sil C 18 W column using a mobile phase of acetonitrile: buffer (50: 50), pH 3,
at a flow rate of 1 mL/min with detection of analyte at 272 nm. The separation was achieved within 3.1 ± 0.3 min for doxofylline
sample. The method showed good linearity in the range of 10–80 μg/mL. The intra and inter day RSD ranged from 0.37–0.53%.
The recovery (mean ± S.D.) of low, middle and high concentrations were 100.04 ± 0.80, 100.01 ± 0.20, 100.07 ± 0.30 respectively.
Limit of detection and limit of quantification were 0.03 and 0.1 μg/mL, respectively. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|