Effects of Sucrose and Trehalose on Stability,Kinetic Properties,and Thermal Aggregation of Firefly Luciferase |
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| Authors: | Sanaz Rasouli Saman Hosseinkhani Parichehreh Yaghmaei Azadeh Ebrahim-Habibi |
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| Affiliation: | (1) Department of Biology, College of Basic Sciences, Science and Research Branch, Islamic Azad University, Pounak, Tehran, Iran;(2) Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran;(3) Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran; |
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| Abstract: | In this study, we used sugars as stabilizing additives to improve the thermostability and to inhibit aggregation of firefly luciferase. The combination of sucrose and trehalose has a strong stabilizing effect on firefly luciferase activity and prevents its thermoinactivation. These additives can also increase optimum temperature. It has been shown that the presence of both sucrose and trehalose can inhibit thermal aggregation of firefly luciferase and decrease bioluminescence decay rate. In order to understand the molecular mechanism of thermostabilization, we investigated the effects of sucrose and trehalose combination on the secondary structure of luciferase by Fourier transform infrared spectroscopy. Minor changes in content of secondary structure of firefly luciferase are observed upon treatment with additives. |
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