Biodegradation of Polyhydroxyalkanoate Films in Natural Environments |
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Authors: | Anatoly N Boyandin Valentin P Rudnev Vladimir N Ivonin Svetlana V Prudnikova Ksenia I Korobikhina Maxim L Filipenko Tatiana G Volova Anthony J Sinskey |
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Institution: | 1. Institute of Biophysics SB RAS. 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia;2. The Joint Vietnam-Russian Tropical Research and Technological Centre, Nguyen Van Huyen, Nghia Do, Cau Giay, Hanoi, Vietnam;3. Siberian Federal University. 79 Svobodnyi Av., Krasnoyarsk, 660041, Russia;4. Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrentiev Ave., Novosibirsk, 630090, Russia;5. Institute of Biophysics SB RAS. 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia
Siberian Federal University. 79 Svobodnyi Av., Krasnoyarsk, 660041, Russia;6. Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA |
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Abstract: | Summary: Biodegradation of film specimens from polyhydroxyalkanoates (PHAs) of two types – poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) – was analysed in different environments: tropical sea waters of the South China Sea (Nha Trang, Vietnam) and soils in the environs of Hanoi (Vietnam), Nha Trang (Vietnam) and Krasnoyarsk (Siberia, Russia). In seawater, the mass loss of the specimens of both types was almost equal. However, in tropical soils, PHB degraded quicker than PHBV. In the Siberian soil, the degradation rate of the PHBV was generally higher than that of PHBV. Analysis of molecular mass of PHA specimens showed its decreasing during biodegradation. In the tropical sea conditions, PHA degrading microorganisms were represented by bacteria of Enterobacter, Bacillus and Gracilibacillus genera. Among PHA degrading bacteria, Burkholderia, Alcaligenes, Bacillus, Mycobacterium and Streptomyces genera were identified in Vietnamese soils, and Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus and Xanthomonas genera in Siberian soils. Micromycetes of Gongronella, Paecilomyces, Penicillium and Trichoderma genera exhibited PHA degrading activity in Vietnamese soils, and Paecilomyces, Penicillium, Acremonium, Verticillium and Zygosporium genera – in Siberian soils. |
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Keywords: | biodegradation biopolymers microbial degradation polyhydroxyalkanoates |
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