Spectroscopic study of gamma irradiated bovine hemoglobin |
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| Affiliation: | 1. Radiation Dosimetry Department, National Institute of Standards (NIS), NIS-Tersa Street 12211, Haram-Giza, P.O. Box 136, Egypt;2. Biophysics Department, Faculty of Science, Cairo University, Giza, P.O. Box 12613, Egypt;1. City University of Hong Kong, Kowloon, Hong Kong;2. Chemistry Department, Faculty of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;3. Université Paris-Est, Laboratoire Modélisation et Simulation Multi Echelle, MSME UMR 8208 CNRS, 5 bd Descartes, 77454 Marne-la-Vallée, France;1. Division of Energy Storage, Dalian National Laboratory of Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;2. University of Chinese Academy of Sciences, Beijing 100039, China;1. Institute of Analytical Science, School of Science, Xi’an Jiaotong University, Xi’an 710049, China;2. College of Pharmacy, Xi’an Jiaotong University, Xi’an 710061, China;3. College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China;1. Department of Pharmaceutical Biotechnology, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune, Maharashtra 411 038, India;2. Department of Pharmacology, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune, Maharashtra 411 038, India;3. Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University, Pune, Maharashtra 411 043, India;4. Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra 411 008, India |
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| Abstract: | In the present study, the effects of ionizing radiation of Cs-137 and Co-60 from 4.95 to 743.14 Gy and from 40 Gy to 300 kGy, respectively, on some bovine hemoglobin characteristics were studied. Such an effect was evaluated using electron paramagnetic resonance (EPR) spectroscopy, and infra-red (IR) spectroscopy. Bovine hemoglobin EPR spectra were recorded and analyzed before and after irradiation and changes were explained in detail. IR spectra of unirradiated and irradiated Bovine hemoglobin were recorded and analyzed also. It was found that ionizing radiation may lead to the increase of free radicals production, the decrease in α-helices contents, which reflects the degradation of hemoglobin molecular structure, or at least its incomplete performance. Results also show that the combined application of EPR and FTIR spectroscopy is a powerful tool for determining structural modification of bovine hemoglobin samples exposed to gamma irradiation. |
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