EPR spin probe investigation of irradiated wheat,rice and sunflower seeds |
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| Institution: | 1. Department of Physics, Faculty of Art and Science, Zonguldak Karaelmas University, 67100 Zonguldak, Türkiye;2. Department of Physics Engineering, Hacettepe University, 06532 Beytepe, Ankara, Türkiye;1. Japan Atomic Energy Agency, 801-1, Mukoyma, Naka-shi, Ibaraki, Japan;2. ITER Organization, Route de Vinon-sur-Verdon, CS 90 046, 13067 St. Paul Lez Durance Cedex, France;3. NIIEFA, 3 doroga na Metallostroy, Metallostroy, St. Petersburg 196641, Russia;1. National Fusion Research Institute, Daejeon, Republic of Korea;2. Hanyang University, Seoul, Republic of Korea;3. Pusan National University, Pusan, Republic of Korea;1. National Institute of Radiological Sciences, 4-9-1, Anagawa, Inage-ku, Chiba-shi 2638555, Japan;2. Japan Wildlife Research Center, 3-3-7, Koutoubashi, Sumida-ku, Tokyo 1308606, Japan;2. Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, 11719 Cairo, Egypt;1. Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita-ku, Sapporo 060-0812, Japan;2. Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, Japan |
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| Abstract: | TEMPO and 4-nitro-TEMPO spin probes were used to monitor dose-dependent changes in the EPR spectra of irradiated wheat and rice embryos and sunflower embryo parts. Rice embryos were studied in the 233–293 K temperature range using 4-nitro-TEMPO. TEMPAMINE, TEMPYO and DTBN spin probes were also studied for their applicability in the determination of irradiated seeds. All the recorded spectra were simulated, and spectral parameters and partition of the probes among various domains were determined. Despite the contribution of the signal from extracellular regions, it was possible to detect the changes in the water/lipid ratios with dose. The hydrophilic character of the probe alone was not sufficient to distinguish the different doses of irradiation. Line widths and rotational correlation times of various domains within embryo also play an important role. Partition after dehydration was another measure in the selection of the suitable probes for irradiation studies. Better results were obtained in dehydrated embryos for the probes preferring lipid bodies. |
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