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The response characteristics of tetrazolium violet solutions to gamma irradiation
Institution:1. Radiation Protection Institute, Ghana Atomic Energy Commission, P.O. Box LG 80, Legon—Accra, Ghana;2. Institute of Isotopes, Hungarian Academy of Sciences, P.O. Box 77, Budapest H-1525, Hungary;3. Department of Physics, University of Cape Coast, Cape Coast, Ghana;1. Hungarian Academy of Sciences, Institute of Isotopes, Budapest, Hungary;2. Hungarian Academy of Sciences, Institute of Isotopes, Budapest, Hungary;1. Biophysics Branch, Physics Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt;2. National Center for Radiation Research and Technology, Atomic Energy Authority (AEA), P. O. Box 8029, Nasr City, Cairo, Egypt;3. Medical Physics Unit, Ain-Shams Hospital, Ain-Shams University, El Abbasia, Cairo, Egypt;1. Physics Department, Faculty of Science, Yarmouk University, Irbid, 21163, Jordan;2. Medical Imaging Department, Faculty of Allied Health Sciences, Hashemite University, Zarqa, 13115, Jordan;3. Ministry of Education, Amman, Jordan;4. Physics Department, Faculty of Science, Hashemite University, Zarqa, Jordan;5. Radiotherapy Department, King Hussein Cancer Center, Amman, Jordan;6. Biomedical Engineering Department, Faculty of Engineering, Hashemite University, Zarqa, 13115, Jordan
Abstract:The dosimetry characteristics of various solutions of tetrazolium violet, TV, (2,5-diphenyl-3-(1-naphthyl)-2H-tetrazolium chloride) to gamma irradiation are reported. The optical absorption spectra of these solutions show peaks between 400 and 600 nm with a shoulder at around 550 nm. The dose response of the optical absorbance values of aqueous and aqueous-alcoholic solutions containing different concentrations of TV was measured in the 250 Gy up to 75 kGy dose range. The formation of formazan product was observed due to radiolytic reduction in both solutions. Its formation was found more pronounced in N2-saturated as well as in alkaline solutions. The results indicate that the 1 mM TV solution can be used for food irradiation and medical sterilization dosimetry at gamma irradiation facilities.
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