Tailoring of the external and internal morphology of poly-3-hydroxy butyrate microparticles |
| |
| Institution: | 1. State University of the North Fluminense. Center of Science and Technology, Campos dos Goytacazes, Ave, Alberto Lamego, 2000 Horto, Brazil;2. Biophysical Institute ‘Carlos Chagas’, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;3. Faculty of Chemistry, University of Havana, Havana, Cuba;1. Instituto Mexicano del Petróleo, Dirección de Investigación en Transformación de Hidrocarburos, Eje Central Lázaro Cárdenas 152, 07730 México D.F., Mexico;2. Institute of Physics and Mechatronics, University of Pannonia, PO Box 158, Veszprém H-8201, Hungary;3. Área de Física de Procesos Irreversibles, División de Ciencias Básicas e Ingeniería, Universidad Autónoma Metropolitana, Av. San Pablo 180 Col. Reynosa, 02200 México D.F., Mexico;1. Cranfield Forensic Institute, Cranfield University, Defence Academy of the United Kingdom, Shrivenham SN6 8LA, United Kingdom;2. Center for Defence Chemistry, Cranfield University, Defence Academy of the United Kingdom, Shrivenham SN6 8LA, United Kingdom;1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China;2. Department of Neonatology, Shanghai Children''s Hospital, Shanghai Jiao Tong University, Shanghai 200040, China;3. Shanghai Institute of Pharmaceutical Industry, Shanghai 200437, China;1. Physics Department, University of the West Indies, P.O. Box 64, Bridgetown, Barbados;2. Centre for Advanced Microscopy (CFAM), University of Reading, Reading RG6 6AD, UK;3. Centro Empresarial da Marinha Grande Rua de Portugal-Zona Industrial, 2430-028 Marinha Grande, Portugal;1. Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan;2. Department of Chemistry, Faculty of Science, The University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan;3. Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Aichi 464-8602, Japan;4. Department of Chemistry, Faculty of Science, Kanagawa University, 2946 Tsuchiya, Hiratsuka, Kanagawa 259-1293, Japan |
| |
| Abstract: | Some microencapsulation procedures such as oil-in-water (o/w) and water-in-oil-in-water (w/o/w) emulsions were selected in an attempt to produce tailored poly-3-hydroxybutyrate (PHB) microparticles. The effects of several processing parameters such as polymer precipitation, surfactant, solvent, stirring and solvent evaporation rates were also considered. As a rule, low stirring rates at 500 rpm yielded particles ranging between 100 to 250 μm and at rates over 8000 rpm, diameters around 5–10 μm. The surfaces of the bigger particles, observed by scanning electron microscopy (SEM), were rough and the smaller ones were even rougher, irregular, cauliflower like. The extraction of the chloroform under low pressure or to the open atmosphere did not produce any appreciable change in the morphology for either type of particle. Transmission electron microscopy (TEM) micrographs suggest that microparticles obtained by o/w emulsions are monolithics but those obtained by w/o/w emulsions are of capsule-like structure. Microencapsulation of a peptide material such as follicle stimulating hormone was carried out with success using a double emulsion technique. This biomaterial, dissolved in the inner aqueous phase, was able to stabilize the primary emulsion without using a surfactant. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
