Fluorescent properties and resonance energy transfer of 3,4-bis(2,4-difluorophenyl)-maleimide |
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Authors: | Katya P Nacheva William A Maza David Z Myers Frank R Fronczek Randy W Larsen Roman Manetsch |
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Institution: | Department of Chemistry, University of South Florida, 4202 East Fowler Ave, CHE 205, Tampa, FL 33620, USA. manetsch@.usf.edu. |
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Abstract: | A fluorescent compound 3,4-bis(2,4-difluorophenyl)-maleimide from the 3,4-diaryl-substituted maleimides was synthesized and determined to have a Stokes shift of 140 nm (λ(abs) 341 nm, λ(em) 481 nm), a high fluorescent quantum yield (Φ(fl) 0.61) and an extinction coefficient ε((340)) of 48?400 M(-1) cm(-1) in dichloromethane. For the first time we demonstrated the successful implementation of a 3,4-diaryl-substituted maleimide molecule as a donor component in FRET experiments. |
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