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Fluorescent properties and resonance energy transfer of 3,4-bis(2,4-difluorophenyl)-maleimide
Authors:Katya P Nacheva  William A Maza  David Z Myers  Frank R Fronczek  Randy W Larsen  Roman Manetsch
Institution:Department of Chemistry, University of South Florida, 4202 East Fowler Ave, CHE 205, Tampa, FL 33620, USA. manetsch@.usf.edu.
Abstract:A fluorescent compound 3,4-bis(2,4-difluorophenyl)-maleimide from the 3,4-diaryl-substituted maleimides was synthesized and determined to have a Stokes shift of 140 nm (λ(abs) 341 nm, λ(em) 481 nm), a high fluorescent quantum yield (Φ(fl) 0.61) and an extinction coefficient ε((340)) of 48?400 M(-1) cm(-1) in dichloromethane. For the first time we demonstrated the successful implementation of a 3,4-diaryl-substituted maleimide molecule as a donor component in FRET experiments.
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