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A mechanically controlled indicator displacement assay |
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| Authors: | Keita Sakakibara Leo A Joyce Taizo Mori Takuya Fujisawa Shagufta H Shabbir Jonathan P Hill Eric V Anslyn Katsuhiko Ariga |
| Affiliation: | World Premier International (WPI) Research Centre for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Ibaraki (Japan) http://www.nims.go.jp/super/HP/E_home.htm; CREST, JST, Sanbancho, Chiyoda-ku102-0075, Tokyo (Japan). |
| Abstract: | Push a host: Mechanical compression was applied to a host monolayer at an interface, which facilitated an indicator displacement assay. The fluorescence resonance energy transfer (FRET) between the host and indicator was switched on by this compression. Addition of D-glucose caused the indicator to be displaced, effectively quenching the FRET process. |
| Keywords: | air–water interface FRET indicator displacement assay molecular recognition sensors |
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