| A chemical labeling of N6-formyl adenosine（f6A） RNA |
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| 作者姓名： | Li-Jun Xie Cui-Lian Lin Li Liu Liang Cheng |
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| 作者单位： | 1. Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences;2. University of Chinese Academy of Sciences |
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| 基金项目： | supported by the National Key R&D Program of China (Nos. 2017YFA0208100 and 2020YFA0707901)；;National Natural Science Foundation of China (Nos. 22022704, 91853124,21977097 and 21778057)； |
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| 摘 要： | N6-methyl adenosine（m6A） is an eminent epigenetic mark in m RNAs that affects a broad range of biological functions in diverse species. However, the chemically inert methyl group prevents a direct labeling of this modification for subsequent detection and sequencing. Therefore, most current approaches for the labeling of m6A still have limitations of relying on the utilization of corresponding methyltransferases,which resulted in the lacking of efficiency. Here w...
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| 收稿时间： | 1 July 2021 |
A chemical labeling of N6-formyl adenosine (f6A) RNA |
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| Li-Jun Xie,Cui-Lian Lin,Li Liu,Liang Cheng.A chemical labeling of N6-formyl adenosine (f6A) RNA[J].Chinese Chemical Letters,2022,33(3):1563-1566. |
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| Institution: | 1. Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | N6-methyl adenosine (m6A) is an eminent epigenetic mark in mRNAs that affects a broad range of biological functions in diverse species. However, the chemically inert methyl group prevents a direct labeling of this modification for subsequent detection and sequencing. Therefore, most current approaches for the labeling of m6A still have limitations of relying on the utilization of corresponding methyltransferases, which resulted in the lacking of efficiency. Here we present an approach which selectively alkylated the N6-formyl adenosine (f6A), the key intermediate during chemical oxidation of m6A, with an alkyne functionality that can be further labeled with click reactions. This covalent labeling approach will be able to facilitate in the affinity purification, detection and genome-wide profiling studies. |
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