A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection |
| |
| Institution: | 1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;2. Hunan Provincial Key Lab of Dark Tea and Jin-Hua, School of Materials and Chemical Engineering, Hunan City University, Yiyang 413000, China;3. Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China;4. Medical School of Ningbo University, Ningbo 315211, China |
| |
| Abstract: | In this work, a simple gold nanoparticles (AuNPs) based colorimetric biosensor was developed for chlorpyrifos (Chl) detection using an aptamer as the capture probe. The Chl-aptamer with low dissociation constant (Kd) of 58.59 ± 6.08 nmol/L was selected by ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for AuNPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately, thus a self-aggregation of AuNPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the LOD as low as 14.46 nmol/L. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, the excellent recoveries with acceptable RSD values below 5% demonstrated that the method can be considered as a promising tool for simple, rapid Chl detection. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
