Recent advances in droplet microfluidics for microbiology |
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| Institution: | 1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China;2. Interdisciplinary Sciences Institute, Huazhong Agricultural University, Wuhan 430070, China;3. State Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China;4. Hubei Hongshan Laboratory, Wuhan 430070, China;5. The Cooperative Innovation Center of Sustainable Pig Production, Wuhan 430070, China;6. Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology, Wuhan 430070, China;7. Institute of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China |
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| Abstract: | Advances in microbiology rely on innovations in technology. Droplet microfluidics, as a versatile and powerful technique that allows high-throughput generation and manipulation of subnanoliter volume droplets, has become an indispensable tool shifting experimental paradigms in microbiology. Droplet microfluidics has opened new avenues to various microbiological research, from resolving single-cell heterogeneity to investigating spatiotemporal dynamics of microbial communities, from precise quantitation of microbiota to systematic decipherment of microbial interactions, and from isolating rare and uncultured microbes to improving genetic engineered strains. In this review, we present recent advances of droplet microfluidics in various fields of microbiology: i) microbial cultivation, ii) microorganism detection and characterization, iii) antibiotic susceptibility testing, iv) microbial interactions, v) microbial biotechnology. We also provide our perspectives on the challenges and future directions for droplet microfluidic-based microbiology research. |
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