| Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin Ⅰ in whole blood samples |
| |
| 作者姓名： | Huan Zhao Enben Su Li Huang Yunfeng Zai Yuan Liu Zhu Chen Song Li Lian Jin Yan Deng Nongyue He |
| |
| 作者单位： | 1. State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University;3. Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology |
| |
| 基金项目： | financially supported by National Natural Science Foundation of China (Nos. 81902153, 61871180, 62071119 and 61971187)；;Jiangsu Provincial Key Research and Development Program (Nos. BA2020016 and BE 2018695)； |
| |
| 摘 要： | Chemiluminescence immunoassay(CLⅠA) has always been a great challenge in detecting cardiac troponin Ⅰ(c Tn Ⅰ) in whole blood samples without centrifugation because of the interference of red blood cells and low sensitivity. Ⅰn this study, the antigens and erythrocytes in the blood were captured by the antibodies immobilized on the magnetic particles, recognized by another biotinconjugated c Tn Ⅰ antibody and detected by streptavidin/acridine aster-conjugated polychloromethylstyrene microspheres(...
|
| 收稿时间： | 25 May 2021 |
Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin I in whole blood samples |
| |
| Huan Zhao,Enben Su,Li Huang,Yunfeng Zai,Yuan Liu,Zhu Chen,Song Li,Lian Jin,Yan Deng,Nongyue He.Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin I in whole blood samples[J].Chinese Chemical Letters,2022,33(2):743-746. |
| |
| Institution: | 1. State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;2. Getein Biotechnology Co., Ltd., Nanjing 210000, China;3. Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China |
| |
| Abstract: | Chemiluminescence immunoassay (CLIA) has always been a great challenge in detecting cardiac troponin I (cTnI) in whole blood samples without centrifugation because of the interference of red blood cells and low sensitivity. In this study, the antigens and erythrocytes in the blood were captured by the antibodies immobilized on the magnetic particles, recognized by another biotin-conjugated cTnI antibody and detected by streptavidin/acridine aster-conjugated polychloromethylstyrene microspheres (PCMS). After magnetic separation, the supernatant was transferred and measured. No significant difference was noted between the cTnI concentrations of the serum samples, plasma samples and whole blood. The prepared PCMS provided more functional areas to conjugate streptavidin and acridinium ester, so the immunoassay has highly sensitive, the limits of blank at 0.012 ng/mL, and functional sensitivity at 0.019 ng/mL with a CV of 20%, and 0.058 ng/mL with a CV of 10%. Total precision of any sample type ranged from 2.62%~5.67%. The assay was linear over the studied range of 0.01–50.00 ng/mL, and no hook effect was found when cTnI concentrations reached 1900 ng/mL. No significant interference was noted with the potential endogenous interfering substances. Compared with the commercial kit (Abbott assay kit), the correlation coefficient was 0.9859. A washing-free CLIA was established for the rapid detection of cTnI in human whole blood, using erythrocyte capture antibodies-conjugated magnetic nanoparticles for eliminating the influence of erythrocytes and PCMS for signal amplification, which showed great potential in clinical application. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
