| DNA origami-based nano-hunter enriches low-abundance point mutations by targeting wild-type gene segments |
| |
| 作者姓名： | Longjie Li Kejun Dong Xinyu Wang Meizhou Zhang Jun Li Andreas K.Nussler Xianjin Xiao Hongbo Wang Yuzhou Wu |
| |
| 作者单位： | 1. School of Life Science and Technology, Wuhan Polytechnic University;2. Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology;3. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology;4. Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology;5. Department of Breast Surgery, Second Hospital of Jilin University;6. Siegfried Weller Institute for Trauma Research, Eberhard-Karls-Universit?t Tübingen, Schnarrenbergstr.95 |
| |
| 基金项目： | funded by the National Key R&D Program of China (Nos. 2018YFA0903500, 2018YFC0114600)；;the National Natural Science Foundation of China (Nos. 51703073, 22077042)；;the Fundamental Research Funds for the Central University (No.2021yjs CXCY114, China)； |
| |
| 摘 要： | Point mutations can be used as biomarkers to perform diagnosis for diseases. In this study, a nanorobot for low-abundance point mutation enrichment was constructed using DNA origami. The novel design achieved limits of detection of 0.1% and 1% for synthesized DNA samples and clinical gene samples, respectively. Resettability was a key property of this method, which also involved a simpler process, lower cost and shorter detection duration than traditional enrichment methods. This novel DNA nanor...
|
| 收稿时间： | 7 August 2021 |
DNA origami‐based nano‐hunter enriches low‐abundance point mutations by targeting wild-type gene segments |
| |
| Longjie Li,Kejun Dong,Xinyu Wang,Meizhou Zhang,Jun Li,Andreas K.Nussler,Xianjin Xiao,Hongbo Wang,Yuzhou Wu.DNA origami-based nano-hunter enriches low-abundance point mutations by targeting wild-type gene segments[J].Chinese Chemical Letters,2022,33(4):2052-2056. |
| |
| Institution: | 1. School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China;2. Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China;3. Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;4. Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;5. Department of Breast Surgery, Second Hospital of Jilin University, Changchun 130041, China;6. Siegfried Weller Institute for Trauma Research, Eberhard-Karls-Universität Tübingen, Schnarrenbergstr. 95, D-72076, Tübingen, Germany |
| |
| Abstract: | Point mutations can be used as biomarkers to perform diagnosis for diseases. In this study, a nanorobot for low-abundance point mutation enrichment was constructed using DNA origami. The novel design achieved limits of detection of 0.1% and 1% for synthesized DNA samples and clinical gene samples, respectively. Resettability was a key property of this method, which also involved a simpler process, lower cost and shorter detection duration than traditional enrichment methods. This novel DNA nanorobot may enable the detection of tumor markers, potentially facilitating early cancer diagnosis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
