A note on the analysis of ligand binding by the 'double-logarithmic' plot |
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| Authors: | Grossweiner L I |
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| Institution: | Wenske Laser Center, Advocate Ravenswood Hospital Medical Center, Chicago, IL 60640, USA. lgflash@aol.com |
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| Abstract: | The double-logarithmic plot has been employed for the fluorometric analysis of ligand binding. This analysis is valid only for 1:1 binding of a non-aggregating ligand and if the plot is based on the free, and not the total, ligand concentration. |
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