高效液相色谱法测定化妆品中氯噻酮和吩噻嗪

建立了反相高效液相色谱法测定化妆品中氯噻酮和吩噻嗪的分析方法。化妆品中氯噻酮和吩噻嗪用丙酮超声提取,采用Kromasil C18色谱柱(5μm,250 mm×4.6 mm i.d.),以30 mmol/L NaH2PO4缓冲液(pH 5.6)和甲醇为流动相进行梯度洗脱,得到了理想的分离效果。氯噻酮和吩噻嗪在0.2~100 mg/L范围内,质量浓度与其峰面积呈良好的线性关系。在低、中、高3个添加水平下,氯噻酮的平均回收率为94.8%~104.1%,RSD为0.7%~4.4%,吩噻嗪的平均回收率为92.5%~103.1%,RSD为1.0%~6.9%。方法可用于化妆品中氯噻酮和吩噻嗪的检测。

Determination of chlortalidone and phenothiazine in cosmetics by high performance liquid chromatography

A method for the determination of chlortalidone and phenothiazine in cosmetics using ultrasonic extraction coupled with high performance liquid chromatography was developed. Chlortalidone and phenothiazine in cosmetics were extracted by ultrasonic extraction with acetone. The extracts were analyzed with HPLC on a kromasil C_(18) column (5 /urn, 250 mm×4.6 mm i.d.) using 30 mmol/L sodium dihydrogen phosphate solution (pH 5.6)-methanol as the mobile phase. Standard curves showed good linearity when the concentrations of chlortalidone and phenothiazine ranged from 0.2 mg/L to 100 mg/L. Under the optimized conditions, recovery studies were performed at three spiking concentrations . The average recoveries of chlortalidone ranged from 94.8 % to 104.1 % with relative standard deviations between 0.7% and 4.4%. The average recoveries of phenothiazine ranged from 92.5% to 103.1% with relative standard deviations between 1.0% and 6.9% . The method has been successfully applied to the determination of chlortalidone and phenothiazine in cosmetics. It is a simple, rapid, accurate method.