Widening the range of high-precision quantitative measurement in retardation-modulated differential interference microscope

To realize high-precision and wide-field measurements in retardation-modulated differential interference contrast (RM-DIC) microscope, we developed a new technique to stitch plural images taken by a high-magnification objective lens with attention paid to the high edge detection ability of the RM-DIC microscope. We showed some experimental results with grating samples and made sure that the developed new method had good performance for widening the measurement field of the RM-DIC microscope.