High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging |
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Authors: | Requejo-Isidro J McGinty J Munro I Elson D S Galletly N P Lever M J Neil M A A Stamp G W H French P M W Kellett P A Hares J D Dymoke-Bradshaw A K L |
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Affiliation: | Department of Physics, Division of Investigative Sciences and Department of Bioengineering, Imperial College London, London, UK. j.requejo@imperial.ac.uk |
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Abstract: | We report the development of a high-speed wide-field fluorescence-lifetime imaging (FLIM) system that provides fluorescence-lifetime images at rates of as many as 29 frames/s. A FLIM multiwell plate reader and a potentially portable FLIM endoscopic system operating at 355-nm excitation have been demonstrated. |
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