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High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging
Authors:Requejo-Isidro J  McGinty J  Munro I  Elson D S  Galletly N P  Lever M J  Neil M A A  Stamp G W H  French P M W  Kellett P A  Hares J D  Dymoke-Bradshaw A K L
Institution:Department of Physics, Division of Investigative Sciences and Department of Bioengineering, Imperial College London, London, UK. j.requejo@imperial.ac.uk
Abstract:We report the development of a high-speed wide-field fluorescence-lifetime imaging (FLIM) system that provides fluorescence-lifetime images at rates of as many as 29 frames/s. A FLIM multiwell plate reader and a potentially portable FLIM endoscopic system operating at 355-nm excitation have been demonstrated.
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