Os(bpy)2(CO)(enIA)][OTf]2: a novel sulfhydryl-specific metal-ligand complex |
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Authors: | Garino Claudio Ghiani Simona Gobetto Roberto Nervi Carlo Salassa Luca Ancarani Valentina Neyroz Paolo Franklin Laurie Alexander Ross J B Seibert Eleanore |
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Affiliation: | Dipartimento di Chimica IFM, Università di Torino, Via P. Giuria 7, 10125 Torino, Italy. |
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Abstract: | The synthesis and physical-chemical characterization of the metal-ligand complex [Os(bpy)2(CO)(enIA)][OTf]2 (where enIA = ethylenediamine iodoacetamide) with a sulfhydryl-specific functional group is described. The UV and visible absorption and luminescence emission, including lifetime and steady-state anisotropy, are reported for the free probe and the probe covalently linked to two test proteins. The spectroscopic properties of the probe are unaffected by chemical modification and subsequent covalent linkage to the proteins. The luminescence lifetime in aqueous buffer is approximately 200 ns and the limiting anisotropy is greater than 0.125, suggesting a potentially useful probe for biophysical investigations. |
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