Effects of chinonin on nitric oxide free radical, myocardial damage and arrhythmia in ischemic-reperfusion injury in vivo

With sodium nitrite NaNO₂ as a standard source of nitric oxide, we compared the correlation coefficients obtained by three measuring methods used currently in the determination of the NOFe²⁺ (DETC)₂ complex (DETC, N,N-diethyldithiocarbamate) with that of the measuring method suggested in this study. The result showed that measuring the total height of triplet signals was the best linear correlation to the concentration of NO compared with other methods used in this system. With this method, we observed the effect of chinonin on the NOFe²⁺ (DETC)₂ complex in myocardial ischemic-reperfusion injury in vivo. The hearts of Wistar rats were subjected to 30 min of ischemia and 10 min of reperfusion in vivo. Different doses of chinonin (5, 10, 25, 50 mg/kg intraperitoneally) were administered to the ischemic-reperfusion rats. Chinonin increased the signal intensity of the NOFe²⁺ (DETC)₂ complex, inhibited the formation of thiobarbituric acid reaction substance and release of creatine kinase, and mitigated the incidence of ventricular arrhythmia in a dose-dependent way. Chinonin has cardiovascular protective effects by means of adjusting the level of NO and inhibiting oxygen free-radical-induced lipid peroxidation in myocardial ischemic-reperfusion injury in vivo.