反相液相色谱中保留值变化规律的研究: 同系物和柱温对保留值的影响

根据反相液相色谱保留模型和Martin方程, 采用热力学方法导出了一组包括各种色谱参数(如柱温、流动相中有机改性剂浓度以及溶质、溶剂和键合相表面烷基配体中的碳数)的线性保留方程。利用这组方程能够解释反相液相色谱中各种线性保留规律和实验现象, 并预测和实验验证了多种文献未报道的线性规律。

Investigations of retention rule in reversed-phase liquid chromatography---Effect of homologous series and column temperature on retention

A few linear retention equations including the various chromatographic parameters (for example,column temperature, composition of mobile phase, number of carbon atoms within the solute molecules, solvent molecules and alkyl ligand on bond phase surface) have been derived by using thermodynamic method based on both the displacement adsorption multi - interaction model in reversed - phase liquid chromatography and the Martin equation. Both the linear retention rules and the experimental phonomena could be explained by these equations,and other linear retention rules which were not reported in the literature have also been predicted and verified experimentally.