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Echo-Induced EPR Spectra of Spin Probes as a Method for Identification of Glassy States in Biological Objects
Institution:1. National High Magnetic Field Laboratory, University of Florida, Gainesville, FL, USA;2. Paul Scherrer Institute, CH-5232 Villigen, Switzerland;3. Center for Hyperpolarization in Magnetic Resonance, Department of Health Technology, Technical University of Denmark, Building 349, 2800 Kgs Lyngby, Denmark;1. Baltic Sea Center, Stockholm University, SE-106 91 Stockholm, Sweden;2. Department of Environmental Science and Analytical Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden;3. Department of Geological Sciences, Stockholm University, SE-106 91 Stockholm, Sweden;4. Department of Earth Sciences, Oxford University, OX1 3AN Oxford, UK;5. Department of Geosciences, Swedish Museum of Natural History, SE-104 05 Stockholm, Sweden;6. Institute of Biological Problems of the Cryolithozone, Yakutsk 677891, Russia;7. Institute for Natural Sciences of North-Eastern Federal University, Yakusk, Russia;1. Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088 Kazan, Russian Federation;2. Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russian Federation;3. Kazan (Volga region) Federal University, 18 Kremlyovskaya str., 420008 Kazan, Russian Federation
Abstract:The echo-induced spectra of nitroxide radicals trapped in organic glasses change their shapes with increases in the time interval between echo-forming microwave pulses. The changes are assigned to the presence in a disordered state of a special type of orientational motion (libration) of molecules near their equilibrium positions. This is not observed in a crystalline state. It is proposed that this effect determines the phase state of the different components of biological systems. The potential of the method is demonstrated by studying the embryos and endosperm of wheat kernels (Tritium vulgare L.) and various models.
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