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Fluorescence high-performance liquid chromatography of arachidonic, eicosapentaenoic and some other higher fatty acids in ovary of several fishes
Authors:Norio Ichinose   Shigeo Abe   Yusho Akishige   Hiroshi Yoshimura  Kyoko Adachi
Affiliation:(1) Department of Chemistry, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31 Hamamatsu, Japan;(2) The Faculty of Fisheries Nagasaki University, 1-14 Bunkyo-cho, 852 Nagasaki, Japan
Abstract:Summary A highly sensitive and selective fluorescence high-performance liquid chromatography using 9-anthryl-diazomethane as a pre-labelling reagent was applied to the simultaneous microanalysis of arachidonic and eicosapentaenoic acids, and some other higher fatty acids at nanogram level in ovary of several tropical adult fishes taken at Rabaul of New Britain. An existing tendency of arachidonic and eicosapentaenoic acids as precursor of prostaglandins in the generative organ of the fishes, has been revealed.
Fluorescenz-HPLC von Arachidonsäure, Eicosapentansäure und einigen anderen höheren Fettsäuren im Ovarium verschiedener Fischarten
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