A Pyrene-based Highly Selective Turn-on Fluorescent Sensor for Copper(II) Ion and its Application in Live Cell Imaging |
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| Authors: | Shu-Pao Wu Zhen-Ming Huang Shi-Rong Liu Peter Kun Chung |
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| Institution: | (1) Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 300, Republic of China |
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| Abstract: | A new pyrene derivative (chemosensor 1) containing a picolinohydrazide moiety exhibits high selectivity for Cu2+ ion detection in mixed aqueous media (CH3OH:H2O = 7:3). Significant fluorescence enhancement was observed with chemosensor 1 in the presence of Cu2+. However, the metal ions Ag+, Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Fe3+, Hg2+, K+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+ produced only minor changes in fluorescence for the system. The apparent association constant (K
a) of Cu2+ binding in chemosensor 1 was found to be 2.75*103 M−1. The maximum fluorescence enhancement caused by Cu2+ binding in chemosensor 1 was observed over the pH range 5–8. Moreover, by means of fluorescence microscopy experiments, it is demonstrated that 1 can be used as a fluorescent probe for detecting Cu2+ in living cells. |
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