Determination of clozapine in human plasma by solid-phase extraction and liquid chromatography with amperometric detection |
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Authors: | M. A. Raggi F. Bugamelli R. Mandrioli D. De Ronchi V. Volterra |
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Affiliation: | (1) Dipartimento di Scienze Farmaceutiche, Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy;(2) Istituto di Psichiatria, Università di Bologna, Viale C. Pepoli 5, 40123 Bologna, Italy |
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Abstract: | Summary A sensitive and selective high-performance liquid chromatographic method has been developed for monitoring clozapine levels in human plasma. Chromatography was performed on a reversed-phase column (C8, 150 mm×4.6 mm i.d., 5 μm) with acetonitrile-aqueous sodium acetate solution, 88∶12 (v/v), as mobile phase; the flow rate was 1 mL min−1. Clozapine oxidation at +800 mV was detected amperometrically. Response was linearly dependent on concentration over the range 50–1500 ng mL−1 clozapine in plasma. Sample preparation by solid-phase extraction before HPLC analysis gave high extraction yield (94%). The accuracy and precision of the method were both very good (recovery: 97%;RSD<3.3%). |
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Keywords: | Column liquid chromatography Amperometric detection Solid-phase extraction Plasma Clozapine |
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