Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol |
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| Authors: | Anna Yu Kolosova Sarah De Saeger Liberty Sibanda Ron Verheijen Carlos Van Peteghem |
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| Institution: | (1) Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium;(2) TOXI-TEST NV, Industrielaan 9a, 9990 Maldegem, Belgium;(3) Euro-Diagnostica BV, Beijerinckweg 18, 6827 BN Arnhem, The Netherlands |
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| Abstract: | A multianalyte lateral-flow technique using colloidal gold-labeled monoclonal antibodies was developed for the rapid simultaneous
detection of deoxynivalenol (DON) and zearalenone (ZEA). The results of this qualitative one-step test were interpreted visually.
A very simple and fast sample preparation was used, and the assay procedure could be accomplished within 10 min. When applied
to spiked wheat samples, the technique gave accurate and reproducible results. Cut-off levels of 1500 and 100 μg kg−1 for DON and ZEA, respectively, were observed. The described multianalyte format can be used as a reliable, rapid and cost-effective
on-site screening technique for the simultaneous determination of mycotoxins in grain samples. |
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| Keywords: | Lateral-flow immunoassay Colloidal gold Deoxynivalenol Zearalenone Multianalyte |
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