| 4-(4-羟基丁基－2－炔氧基)－3-（苯磺酰基）－1，2，5－噁二唑－2－氧化物与牛血清白蛋白结合作用的光谱法研究 |
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| 引用本文： | 孙绍发,项光亚,候汉娜,刘义.4-(4-羟基丁基－2－炔氧基)－3-（苯磺酰基）－1，2，5－噁二唑－2－氧化物与牛血清白蛋白结合作用的光谱法研究[J].中国化学,2006,24(8):1050-1053. |
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| 作者姓名： | 孙绍发 项光亚 候汉娜 刘义 |
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| 作者单位： | ~aDepartment of Chemistry and Life Science Xianning College，Xianning，Hubei 43 7005，China ~bDepartment of Chemical Biology，College of Chemistry and Molecular Sciences，Wuhan University，Wuhan，Hubei 430072，China，~dSchool of Pharmacy，Tongji Medical College，Huazhong University of Science and Technology，Wuhan，Hubei 430015，China，Department of Chemical Biology，College of Chemistry and Molecular Sciences，Wuhan University，Wuhan，Hubei 430072，China，Department of Chemical Biology，College of Chemistry and Molecular Sciences，Wuhan University，Wuhan，Hubei 430072，China ~cState Key Laboratory of Virology，Wuhan University，Wuhan，Hubei 430072，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 30570015, 20373051 ）, the Natural Science Foundation of Hubei Province （Nos. 2006ABA333, 2005ABC002）, and the Science Foundation of Hubei Provincial Educational Department （No. D200528003）, and the Science Research Foundation of Chinese Ministry of Education （No. [2006]8IRT0543）. |
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| 摘 要： | The interaction between 4-(4-hydroxybut-2-ynyloxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole-2-oxide(FB)andbovine serum albumin(BSA)was studied by spectroscopic methods including fluorescence and UV-Vis absorptionspectroscopy.The quenching mechanism of fluorescence of BSA by FB was considered to be a dynamic quenchingprocedure.The number of binding sites n and apparent binding constant K were measured by fluorescence quench-ing method.The results indicate that there is FB molecular binding with BSA,and forming 1∶1 complex.Thethermodynamic parameters such as ΔH,ΔG and ΔS,etc.,were calculated.The results indicate that the binding reac-tion is mainly entropy-driven and hydrophobic forces play major role in the binding reaction.The distance r be-tween donor(BSA)and acceptor(FB)was obtained according to Frster theory of non-radioactive energy transfer.
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| 关 键 词： | 荧光淬火 热力学参数 牛血清白蛋白 4-（4-羟基-2-菩他卡因氧）-3-苯基磺酰二价基-1 2 5- 氧杂二唑-2-氧化物 光谱分析 |
| 收稿时间： | 2006-04-15 |
| 修稿时间： | 2006-04-152006-06-10 |
Studies on Interaction between 4-(4-Hydroxybut-2-ynyloxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole-2-oxide and Bovine Serum Albumin by Spectroscopic Method |
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| SUN Shao-Fa, XIANG Guang-ya, HOU Han-Na, LIU Yi.Studies on Interaction between 4-(4-Hydroxybut-2-ynyloxy)-3-(phenylsulfonyl)-1,2,5-oxadiazole-2-oxide and Bovine Serum Albumin by Spectroscopic Method[J].Chinese Journal of Chemistry,2006,24(8):1050-1053. |
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| Authors: | SUN Shao-Fa XIANG Guang-ya HOU Han-Na LIU Yi |
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| Institution: | Department of Chemistry and Life Science, Xianning College, Xianning, Hubei 437005, China ; Department of Chemical Biology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei 430072, China ; State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China ; School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei 430015, China |
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| Abstract: | |
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| Keywords: | fluorescence quenching thermodynamic parameter bovine serum albumin 4-(4-hydroxybut-2-ynyloxy)-3-(phenylsulfonyl)-1 2 5-oxadiazole-2-oxide |
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