Spectral Reproducibility and Quantification of Peptides in MALDI of Samples Prepared by Micro-Spotting |
| |
Authors: | Yong Jin Bae Kyung Man Park Sung Hee Ahn Jeong Hee Moon Myung Soo Kim |
| |
Institution: | 1. Department of Chemistry, Seoul National University, Seoul, 151-747, Korea 2. Medical Proteomics Research Center, KRIBB, Daejeon, 305-806, Korea
|
| |
Abstract: | Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases. Figure ? |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|