| 31ku激发子蛋白的液相色谱分离纯化 |
| |
| 引用本文： | 马志超,楼健.31ku激发子蛋白的液相色谱分离纯化[J].分析测试学报,2002,21(2):22-25. |
| |
| 作者姓名： | 马志超 楼健 |
| |
| 作者单位： | 浙江大学华家池校区分析测试中心,浙江杭州,310029 |
| |
| 基金项目： | 国家自然科学基金资助项目(30170084) |
| |
| 摘 要： | 利用阴离子交换色谱和疏水相互作用色谱从烟草疫霉菌培养液中分离出一种新的31ku激发子蛋白，选择了阴离子交换色谱流动相的的最佳pH值6．0，建立了疏水相互作用色谱硫酸铵Tris缓冲液－水洗脱模式，简化了纯化步骤和减少了活性损失的危险。
|
| 关 键 词： | 阴离子交换色谱 疏水相互作用色谱 31ku激发子蛋白 分离 纯化 疫霉菌 植物病原菌 |
| 文章编号： | 1004-4957(2002)02-0022-03 |
| 修稿时间： | 2001年5月17日 |
Separation and Purification of 31 ku Elicitor by LC |
| |
| MA Zhi _ chao,LOU Jian.Separation and Purification of 31 ku Elicitor by LC[J].Journal of Instrumental Analysis,2002,21(2):22-25. |
| |
| Authors: | MA Zhi _ chao LOU Jian |
| |
| Abstract: | A new 31 ku elicitor from a culture medium of P.parasitica Var. nicotiana was separated and purified by a chromatographic procedure including anion exchange chromatography and hydrophobic interaction chromatography. The chromatographic conditions were studied. The optimum pH value was found to be 6.0 for anion exchange chromatography. An elution mode of Tris buffer of ammonia sulfate-water was established for hydrophobic interaction chromatography. The procedure simplified working process and decreased the activity loss of the elicitor. |
| |
| Keywords: | Anion exchange chromatography Hydrophobic interaction chromatography Elicitor Protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
