Detection of avian influenza virus using an interferometric biosensor |
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Authors: | Jie Xu David Suarez David S. Gottfried |
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Affiliation: | Georgia Tech Research Institute, Georgia Institute of Technology, Atlanta, GA 30332-0801, USA. |
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Abstract: | An interferometric biosensor immunoassay for direct and label-less detection of avian influenza through whole virus capture on a planar optical waveguide is described. The assay response is based on index of refraction changes that occur upon binding of virus particles to unique antigen-specific (hemagglutinin) antibodies on the waveguide surface. Three virus subtypes (two H7 and one H8) in buffer solution were tested using both monoclonal and polyclonal capture antibodies. The real-time response of the antigen-antibody interaction was measured and was shown to be concentration-dependent, with detection limits as low as 0.0005 hemagglutination units per milliliter. A simple sandwich assay was shown to further increase the biosensor response. |
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Keywords: | Optical waveguide Interferometer Immunoassay Antibody Hemagglutinin |
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