Low-temperature enzymatic hydrolysis resolution in mini-emulsion media |
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Authors: | Nuno M T Lourenço Sara C Matias Margarida C Altas Luis P Fonseca |
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Institution: | 1.Centre for Biological and Chemical Engineering,IBB - Institute for Biotechnology and Bioengineering,Lisboa,Portugal;2.Department of Bioengineering,Instituto Superior Técnico,Lisboa,Portugal |
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Abstract: | A low-temperature mini-emulsion medium for the enzymatic resolution of 1-phenylethanol is described for the first time. The enzymatic hydrolysis resolution of 1-phenylethyl esters with different chain-lengths in the presence of Candida antarctica lipase B in mini-emulsion media was shown to be significantly controlled by temperature. In this system, the direct effect of temperature on the mini-emulsion size was observed. For the longer 1-phenylethyl ester, 1-phenylethyl dodecanoate, the enzymatic resolution was promoted exclusively at low temperatures. The preparative mini-emulsion enzymatic reaction of 1-phenylethyl dodecanoate at 4 °C afforded the isolation of (R)-phenylethanol with a yield of 36 % with an ee of 99 %. (S)-Phenylethanol was isolated with a 51 % yield with an ee of 79 %. |
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