Application of polypyrrole nanowires for the development of a tyrosinase biosensor |
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| Authors: | Jolanta Kochana Katarzyna Hnida Grzegorz Sulka Pawe? Knihnicki Joanna Kozak Agnieszka Gilowska |
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| Institution: | 1.Department of Analytical Chemistry, Faculty of Chemistry,Jagiellonian University,Kraków,Poland;2.Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry,Jagiellonian University,Kraków,Poland;3.Academic Centre for Materials and Nanotechnology,AGH University of Science and Technology,Kraków,Poland |
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| Abstract: | Polypyrrole nanowires (PPyNWs) were fabricated and examined as a structural component of amperometric biosensor matrix. An enzyme, tyrosinase (TYR), was immobilized onto PPyNWs using glutaraldehyde (GA). Matrix composite morphology was investigated using scanning electron microscopy. Electrochemical behavior of the prepared PPyNWs/GA/TYR biosensor towards catechol was studied and the assessment of its analytical characteristics was carried out taking into account linear range, sensitivity, repeatability, reproducibility and operational stability. |
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