A Novel Stepwise Recovery Strategy of Cellulase Adsorbed to the Residual Substrate after Hydrolysis of Steam Exploded Wheat Straw |
| |
Authors: | Jian Xu Hongzhang Chen |
| |
Institution: | (1) State Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China;(2) Environmental Science and Forestry, State University of New York, Syracuse, NY 13210, USA |
| |
Abstract: | Cellulase distribution between residual substrate and supernatant in the process of enzymatic hydrolysis of steam-exploded
wheat straw was investigated. Subsequently, a novel stepwise recovery strategy with three successive steps was adopted to
recover cellulase adsorbed to the residual substrate. The results showed that cellulase protein in the supernatant increased
as the hydrolysis time increased. When hydrolysis ended, the cellulase remaining on the residual substrate accounted for 33–42%
of the original added cellulase according to the different cellulase loading. To obtain the maximum cellulase recovery rate,
the residual substrate was dealt with in three successive steps: washed with sodium acetate buffer (step 1), shaken with sodium
acetate buffer (step 2), and then treated with 0.0015 mol/L, pH 10 Ca(OH)2 (step 3). The total cellulase protein recovered by the three steps reached 96.70–98.14%. The enzyme activity of cellulase
recovered by the first two steps was kept well. The ratios of the specific activity between the recovered cellulase and the
original were 89–96%, which was by far higher than that using step 3 (the value was 48% ∼ 56%). |
| |
Keywords: | Cellulase Enzymatic hydrolysis Residual substrate Stepwise recovery strategy Specific activity |
本文献已被 PubMed SpringerLink 等数据库收录! |
|