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Essential Oil Composition of Osmanthus fragrans Varieties by GC-MS and Heuristic Evolving Latent Projections
Authors:Chun-Di Hu  Yi-Zeng Liang  Xiao-Ru Li  Fang-Qiu Guo  Mao-Mao Zeng  Liang-Xiao Zhang  Hong-Dong Li
Institution:1. Research Center of Modernization of Chinese Herbal Medicines, College of Chemistry and Chemical Engineering, Central South University, 410083, Changsha, People’s Republic of China
2. Pharmaceutical School of Xianning University, 437100, Xianning, People’s Republic of China
Abstract:The essential oils from two varieties of Osmanthus fragrans have been extracted by steam distillation and analyzed by gas chromatography-mass spectrometry with the help of heuristic evolving latent projections (HELP), an effective chemometric resolution method. The overlapped peak clusters were resolved into pure chromatograms and pure mass spectra with HELP. Identification of the components was by comparison of temperature-programmed retention indices (PTRIs) and by similarity searches in the National Institute of Standards and Technology mass database. Quantitative results were obtained by overall integration of the peaks. The reliability of the qualitative and quantitative results was greatly improved by using HELP and PTRIs. The main components from O. fragrans Lour. var. thunbergii Mak. (TM) and O. fragrans Lour. var. aurantiacus Mak. (AM) were 1,2-epoxy linalool and nonanal, respectively. In total, 52 volatile components in essential oil of TM and 45 in AM were analyzed qualitatively and quantitatively, accounting for 95.67 and 92.28% total contents of the essential oils.
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