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Determination of Histamine in Rat Plasma and Tissue Extracts by Intramolecular Excimer-Forming Derivatization and LC with Fluorescence Detection |
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| Authors: | Fumio Ichinose Takashi Yoshitake Hideyuki Yoshida Kenichiro Todoroki Jan Kehr Osamu Inoue Hitoshi Nohta Masatoshi Yamaguchi |
| Institution: | 1. Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan-ku, Fukuoka, 814-0180, Japan 2. Chemicals Evaluation and Research Institute, 3-822 Ishii Machi, Hita, Oita, 877-0061, Japan 3. Department of Physiology and Pharmacology, Karolinska Institutet, Nanna Svartz vag 2, 171 77, Stockholm, Sweden 4. School of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara, Tochigi, 324-8501, Japan 5. Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| Abstract: | A highly sensitive and selective method for determination of histamine in rat plasma and tissue extracts by liquid chromatography with fluorescence detection is described. The method is based on precolumn derivatization of amino groups of histamine with two molecules of 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester that allows generation of intramolecular excimer fluorescence. The detection limit for histamine was 0.183 nM. This sensitivity allowed determination of histamine in 10 μL of rat plasma or in the extracts from less than 1 mg of tissue. |
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