Evaluation of Two Techniques for Extraction of Cortisol from Human Saliva |
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Authors: | Ewelina Dziurkowska Marek Wesolowski |
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Institution: | 1. Department of Analytical Chemistry, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland
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Abstract: | The aim of the study was to compare two kinds of extraction of cortisol from human saliva. The first was made with dichloromethane and the second by solid-phase extraction. Saliva was sampled in the evening into plastic tubes then cortisol was extracted and analyzed by LC with detection at 240 nm. The limit of detection was 1 ng mL?1 for extraction with dichloromethane and 3 ng mL?1 for SPE extraction. Both methods of extraction were applied to the analysis of saliva samples without any interference peaks. |
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