Simultaneous Determination of Main Bioactive Components in Rosa multiflora Thunb. and Their Fragmentation Study by LC–MS |
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Authors: | Nannan Pang Dilinur Malike Huwei Liu |
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Institution: | 1. Beijing National Laboratory for Molecular Sciences, the Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People’s Republic of China 2. College of Life and Environmental Sciences, Xinjiang Normal University, 830053, Xinjiang, People’s Republic of China
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Abstract: | Five naturally bioactive compounds, belonging to the representative flavonoid and pentacyclic triterpenoid types, were separated by liquid chromatography and analyzed on-line with negative ion ESI–MS. Rutin, quercetin, and oleanolic acid in the extracts of Rosa multiflora Thunb. of China were identified by the comparison of their retention times and mass spectrometric data with those of authentic standards, and their contents were 0.22, 3.8, and 45 µg g?1 of dry weight in rose hips, respectively. Five standards, including rutin, quercetin, kaempferol, lysionotin, and oleanolic acid were investigated by MS3, which facilitate further understanding of their structures. |
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