Rapid Separation of Hydrophobic Compounds by UPLC: in Case of Bisindolylmaleimides, Indolocarbazoles or Fullerene C60 Derivatives |
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Authors: | Manabu Nakazono Yasuyuki Sato Shigeo Hasegawa Norimasa Arisuda Aiko Miyamoto Kiyoshi Zaitsu |
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Institution: | 1. Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan
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Abstract: | Rapid separation of hydrophobic compounds such as bisindolylmaleimides, indolocarbazoles or fullerene C60 derivatives by ultra performance liquid chromatography was developed. Bisindolylmaleimides and indolocarbazoles were separated within 7 and 5 min, respectively. The linear ranges for the determination of bisindolylmaleimides and indolocarbazoles were 1.25?500 pmol and 0.5?50 pmol per injection (r > 0.99), respectively. Fullerene C60 derivatives were separated within 9 min, and the linear range for the determination of fullerene C60 derivatives was 0.1?10 pmol per injection (r > 0.99). |
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