Chemometric Approach for Simultaneous Optimization of Resolution and Analysis Time in CCC

The chemometric approach combining Box–Behnken response surface model and Derringer’s desirability function was applied for simultaneous optimization of resolution and analysis time in countercurrent chromatography (CCC). The mergence of the two parameters was accomplished using the Derringer’s desirability function with subsequent optimization by a Box–Behnken response surface design. The developed model was checked by statistical analysis. By implementing the optimal flow rate, rotation speed and temperature predicted by the validated model, enhanced resolution between two similar analytes (phenol and resorcinol) was achieved in a reasonable time. The analyses and results obtained in this paper will benefit to improve the efficiency of CCC separation.