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Separation and Purification of Three Flavonoids from Helichrysum arenarium (L.) Moench by HSCCC |
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| Authors: | Yi Yang Yun Huang Dongyu Gu Abulimiti Yili Gulnar Sabir Haji Akber Aisa |
| Affiliation: | 1. Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technological Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, China 2. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China 3. Xinjiang Institute of Chinese Material Medica and Ethica, Urumqi, 830002, China |
| Abstract: | Following an initial clean-up step on a Sephadex LH-20 column, high-speed countercurrent chromatography was successfully applied to the isolation and purification of three flavonoids from a crude sample of Helichrysum arenarium (L.) Moench. HSCCC was performed with a two-phase solvent system composed of ethyl acetate–water (1:1, v/v). Naringenin-7-O-β-d-glycoside (2.3 mg), isoquercitrin (3.5 mg), and astragalin (6.7 mg), with purities of 96.05%, 93.63%, 95.23%, respectively, were separated from 160 mg of crude sample in a one-step separation. The structure identification was by 1H NMR and 13C NMR. |
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