Improvements in confocal microscopy imaging using serrated divided apertures |
| |
| Authors: | Wei Gong Ke Si Colin JR Sheppard |
| |
| Institution: | aDivision of Bioengineering, National University of Singapore, Singapore 117574, Singapore;bNUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117456, Singapore;cDepartment of Biological Sciences, National University of Singapore, Singapore 117543, Singapore |
| |
| Abstract: | Confocal microscopy with serrated divided apertures is presented. Compared with using two smooth D-shaped apertures in confocal microscopy, the serrated divided apertures can suppress the out-of-focus central bright spot, thus increasing the efficiency of rejection of scattered light. Diffraction analysis shows that the serrated apertures maintain the optical sectioning strength while attenuating the background coming from far from the focal plane. In addition, the signal to background ratio (S/B) is also improved. |
| |
| Keywords: | Confocal microscopy Serrated divided apertures Signal to background ratio Angular gating |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
