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AAPBA@KCC-1的合成及其对血清中唾液酸和神经节苷脂的分析应用
引用本文:田华君,薛芸,陈巧梅,王彦,阎超.AAPBA@KCC-1的合成及其对血清中唾液酸和神经节苷脂的分析应用[J].色谱,2017,35(5):473-481.
作者姓名:田华君  薛芸  陈巧梅  王彦  阎超
作者单位:上海交通大学药学院, 上海 200240
基金项目:上海市科委科研计划项目(15142200200,16142200300);中国博士后科学基金(2015M581628);上海市引进技术的吸收与创新专项(XC-ZXSJ-02-2016-11).
摘    要:将3-丙烯酰胺基苯硼酸(AAPBA)修饰在介孔二氧化硅KCC-1的表面,合成AAPBA@KCC-1材料,并通过扫描电子显微镜、透射电子显微镜、N_2吸附解吸附测试和X射线光电子能谱对其进行表征;将AAPBA@KCC-1材料作为分散固相萃取(dSPE)的吸附剂,对血清样品中的唾液酸和神经节苷脂(GLS)进行提取和富集;采用傅里叶变换离子回旋共振质谱法和超高效液相色谱-离子淌度-四极杆飞行时间质谱法分别对AAPBA@KCC-1材料吸附得到的唾液酸和神经节苷脂进行分析。结果表明,AAPBA@KCC-1材料能够有效吸附唾液酸和神经节苷脂,简化了样品前处理的操作,可以进一步应用于生物样品中唾液酸和神经节苷脂的研究。

关 键 词:分散固相萃取  介孔二氧化硅KCC-1  3-丙烯酰胺基苯硼酸  N-乙酰神经氨酸  唾液酸  神经节苷脂
收稿时间:2017-01-20

Synthesis of AAPBA@KCC-1 and its application in the analysis of sialic acid and gangliosides in serum
TIAN Huajun,XUE Yun,CHEN Qiaomei,WANG Yan,YAN Chao.Synthesis of AAPBA@KCC-1 and its application in the analysis of sialic acid and gangliosides in serum[J].Chinese Journal of Chromatography,2017,35(5):473-481.
Authors:TIAN Huajun  XUE Yun  CHEN Qiaomei  WANG Yan  YAN Chao
Institution:School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
Abstract:A new material AAPBA@KCC-1 was synthesized by using 3-acrylamidophenylboronic acid (AAPBA) modified on mesoporous silica KCC-1. First, AAPBA was modified on the surface of KCC-1 by three-step synthesis method. Then, AAPBA@KCC-1 was characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), N2 adsorption-desorption isotherm and X-ray photoelectron spectroscopy (XPS). AAPBA@KCC-1 was used as the dispersive solid-phase extraction (dSPE) adsorbent to extract and enrich sialic acid and ganglioside (GLS) from serum samples. Finally, Fourier transform ion cyclotron resonance mass spectrometry (FT-MS) and ultra performance liquid chromatography-ion mobility spectrometry-quadrupole time of flight mass spectrometry (UPLC-IMS-QTOF MS) were used to evaluated the adsorption ability of AAPBA@KCC-1 to sialic acid and GLS, respectively. The results showed that AAPBA@KCC-1 can effectively adsorb sialic acid and GLS, and can be used for further studies of sialic acid and GLS in biological samples.
Keywords:dispersive solid-phase extraction (dSPE)  mesoporous silica KCC-1  3-acrylamidophenylboronic acid (AAPBA)  N-acetylneuraminic acid (Neu5Ac)  sialic acid  ganglioside (GLS)
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