A Caged In-Source Laser-Cleavable MALDI Matrix with High Vacuum Stability for Extended MALDI-MS Imaging |
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| Authors: | Qiuqin Zhou Stefano Rizzo Janina Oetjen Annabelle Fülöp Miriam Rittner Hartmut Gillandt Carsten Hopf |
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| Institution: | 1. Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany;2. Sirius Fine Chemicals SiChem GmbH, Fahrenheitstr. 1, 28359 Bremen, Germany;3. Bruker Daltonics GmbH & Co. KG, Fahrenheitstr. 4, 28359 Bremen, Germany |
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| Abstract: | Insufficient vacuum stability of matrix chemicals is a major limitation in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) of large tissue sample cohorts. Here, we designed and synthesized the photo-cleavable caged molecule 4,5-dimethoxy-2-nitrobenzyl-2,5-dihydroxyacetophenone (DMNB-2,5-DHAP) and employed it for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP is vacuum-stable in a high vacuum MALDI ion source for at least 72 h. Investigation of the uncaging process suggested that the built-in laser (355 nm) in the MALDI ion source promoted the in situ generation of 2,5-DHAP. A caging group is used for the first time in designing a MALDI matrix that is vacuum-stable, uncaged upon laser irradiation during the measurement process, and that boosts lipid ion intensity with MALDI-2 laser-induced postionization. |
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| Keywords: | Caged Compound MALDI MS Imaging MALDI Matrix Mass Spectrometry Vacuum Stability |
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