MODULATING ACTIVITY OF B LYMPHOCYTES ON HAEMOPOIESIS (Ⅱ)——THE PURIFICATION OF PROLIFERATION AMPLIFYING FACTOR (PAF) |
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作者姓名: | 姚志建 江飞子 黎燕 郭燕捷 张明伟 徐元基 唐佩弦 |
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作者单位: | Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PLA. Beijing,Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PLA. Beijing,Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PLA. Beijing,Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PLA. Beijing,Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PLA. Beijing,Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PLA. Beijing,Institute of Basic Medical Sciences,Academy of Military Medical Sciences. PLA. Beijing |
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基金项目: | Project supported by the National Natural Science Foundation of China. |
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摘 要: | In recent years we have done a series of observations on the haemopoietic modulation effects oflymphocytes. Now we report here, for the first time, the pulification of a modulating factor (prolif-eration amplifying factor, PAE) with affinity chromatography and HPLC from supernatant conditionedby a lymphoma cell line M12.4.1. RAF exhibits on SDS-PAGE only one band, the molecular weightis about 31,000, the specific activity is 20,000 times that in the conditioned medium. It may providea direct evidence that the B lymphocyte also possesses some haemopoietic modulation effects.
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