Two-dimensional diversity: screening human cDNA phage display libraries with a random diversity probe for the display cloning of phosphotyrosine binding domains |
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Authors: | Videlock Elizabeth J Chung Victor K Mohan Michael A Strok Timothy M Austin David J |
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Affiliation: | Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA. |
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Abstract: | A random phosphopeptide probe (bio-pYZZZ) has been used for the isolation and identification of multiple SH2 domains from human cDNA-displaying phage libraries. In addition, on-phage analysis and quantification of binding affinities for these phage-displayed proteins has shown them to be functional domains, retaining the same characteristics as in their native state. |
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