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On site visual detection of <Emphasis Type="Italic">Porphyromonas gingivalis</Emphasis> related periodontitis by using a magnetic-nanobead based assay for gingipains protease biomarkers |
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| Authors: | Sahar?Alhogail Ghadeer?A?R?Y?Suaifan Sergio?Bizzarro Wendy?E?Kaman Floris?J?Bikker Karina?Weber Dana?Cialla-May Jürgen?Popp |
| Institution: | 1.Department of Medical Technology, College of Applied Medical Sciences,King Saud University,Riyadh,Saudi Arabia;2.Department of Pharmaceutical Sciences, Faculty of Pharmacy,The University of Jordan,Amman,Jordan;3.Department of Periodontology, Academic Centre for Dentistry Amsterdam,University of Amsterdam and VU UniversityAmsterdam,Amsterdam,The Netherlands;4.Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam,University of Amsterdam and VU University Amsterdam,Amsterdam,The Netherlands;5.Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center,Rotterdam,The Netherlands;6.Institute of Physical Chemistry and Abbe Center of Photonics,Friedrich Schiller University Jena,Jena,Germany;7.InfectoGnostics Research Campus Jena, Center for Applied Research,Jena,Germany;8.Leibniz Institute of Photonic Technology,Jena,Germany;9.Faisal Specialist Hospital and Research Center,Riyadh,Saudi Arabia;10.Department of Chemistry,Alfaisal University,Riyadh,Saudi Arabia |
| Abstract: | Porphyromonas gingivalis (P. gingivalis) is a pathogen causing periodontitis. A rapid assay is described for the diagnosis of periodontal infections related to P. gingivalis. The method is making use of gingipains, a group of P. gingivalis specific proteases as a detection biomarker. Magnetic-nanobeads were labeled with gingipain-specific peptide substrates and immobilized on a gold biosensing platform via gold-thiol linkage. As a result of this, the color of the gold layer turns black. Upon cleavage of the immobilized substrates by gingipains, the magnetic-nanobeads-peptide fragments were attracted by a magnet so that the golden surface color becomes visible again. This assay is highly sensitive and specific. It is capable of detecting as little as 49 CFU·mL?1 of P. gingivalis within 30 s. Examination of periodontitis patients and healthy control saliva samples showed the potential of the assay. The simplicity and rapidity of the assay makes it an effective point-of-care device. Graphical abstract Schematic of the assay for the detection of P. gingivalis proteases as one of the promising biomarkers associated with periodontal diseases. |
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