Microfluidic capture and release of bacteria in a conical nanopore array |
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Authors: | Guo Peng Hall Eric W Schirhagl Romana Mukaibo Hitomi Martin Charles R Zare Richard N |
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Affiliation: | Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA. |
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Abstract: | We present a simple and inexpensive method for the capture and release of bacteria contained in an array of conical nanopores on a membrane inside a microfluidic device. As an example, we demonstrate that cyanobacteria can be captured, one bacterium per pore, in a defined orientation with over 500 bacteria per membrane with viabilities as high as 100%. The device can also specifically capture cyanobacteria from a mixed suspension of cyanobacteria and chlamydomonas with a selectivity as high as 90%. |
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